The analysis of inflammatory cell migration using primary neutrophils

Introduction Neutrophils are the primary defense of the host against pathogens. They remain in resting state in the circulation of the healthy individuals. When encountering cytokine or chemokine signals caused by pathogen products, neutrophils are activated and mobilized to the infected sites. The life span of neutrophils which have an essential role in terms of pathogenesis of autoinflammatory diseases are very short. In consideration of this issue, it is restricted to study neutrophil migration in vitro. Hence, we developed a new approach on cell migration assays and neutrophil priming for long lasting research.